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Fig. 2. Differentiated osteoblasts demonstrated decreased coagulation parameters compared with MSCs. Immunostaining revealed a significant reduction in the expression of TF, <t>TFPI,</t> heparanase and TFPI-2 in osteoblast cells. Contiguous Tables show the indicated protein staining intensity in cells. Significance was determined using the Mann–Whitney U test. Representative images were visualized at × 10 magnitude, with 0.82 MDC objective lens, captured with a Nikon E995 digital camera (Nikon, Tokyo, Japan), and processed with Adobe Photoshop software (Adobe Systems, San Jose, CA, USA).
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Fig. 2. Differentiated osteoblasts demonstrated decreased coagulation parameters compared with MSCs. Immunostaining revealed a significant reduction in the expression of TF, <t>TFPI,</t> heparanase and TFPI-2 in osteoblast cells. Contiguous Tables show the indicated protein staining intensity in cells. Significance was determined using the Mann–Whitney U test. Representative images were visualized at × 10 magnitude, with 0.82 MDC objective lens, captured with a Nikon E995 digital camera (Nikon, Tokyo, Japan), and processed with Adobe Photoshop software (Adobe Systems, San Jose, CA, USA).
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anti tfpi  (Santa Cruz Biotechnology)
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Fig. 2. Differentiated osteoblasts demonstrated decreased coagulation parameters compared with MSCs. Immunostaining revealed a significant reduction in the expression of TF, <t>TFPI,</t> heparanase and TFPI-2 in osteoblast cells. Contiguous Tables show the indicated protein staining intensity in cells. Significance was determined using the Mann–Whitney U test. Representative images were visualized at × 10 magnitude, with 0.82 MDC objective lens, captured with a Nikon E995 digital camera (Nikon, Tokyo, Japan), and processed with Adobe Photoshop software (Adobe Systems, San Jose, CA, USA).
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anti tfpi 1 polyclonal antibody  (Santa Cruz Biotechnology)
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Fig. 2. Differentiated osteoblasts demonstrated decreased coagulation parameters compared with MSCs. Immunostaining revealed a significant reduction in the expression of TF, <t>TFPI,</t> heparanase and TFPI-2 in osteoblast cells. Contiguous Tables show the indicated protein staining intensity in cells. Significance was determined using the Mann–Whitney U test. Representative images were visualized at × 10 magnitude, with 0.82 MDC objective lens, captured with a Nikon E995 digital camera (Nikon, Tokyo, Japan), and processed with Adobe Photoshop software (Adobe Systems, San Jose, CA, USA).
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Fig. 2. Differentiated osteoblasts demonstrated decreased coagulation parameters compared with MSCs. Immunostaining revealed a significant reduction in the expression of TF, TFPI, heparanase and TFPI-2 in osteoblast cells. Contiguous Tables show the indicated protein staining intensity in cells. Significance was determined using the Mann–Whitney U test. Representative images were visualized at × 10 magnitude, with 0.82 MDC objective lens, captured with a Nikon E995 digital camera (Nikon, Tokyo, Japan), and processed with Adobe Photoshop software (Adobe Systems, San Jose, CA, USA).

Journal: Scientific reports

Article Title: Effect of bone marrow blood versus peripheral blood on the hemostatic balance of osteoblasts and endothelial cells.

doi: 10.1038/s41598-025-94942-x

Figure Lengend Snippet: Fig. 2. Differentiated osteoblasts demonstrated decreased coagulation parameters compared with MSCs. Immunostaining revealed a significant reduction in the expression of TF, TFPI, heparanase and TFPI-2 in osteoblast cells. Contiguous Tables show the indicated protein staining intensity in cells. Significance was determined using the Mann–Whitney U test. Representative images were visualized at × 10 magnitude, with 0.82 MDC objective lens, captured with a Nikon E995 digital camera (Nikon, Tokyo, Japan), and processed with Adobe Photoshop software (Adobe Systems, San Jose, CA, USA).

Article Snippet: Polyclonal anti-TF, polyclonal anti-TFPI antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Coagulation, Immunostaining, Expressing, Staining, MANN-WHITNEY, Software

Fig. 8. Graphical summarization. Levels of heparanase, TF, TFPI, and TFPI-2 were reduced in osteoblasts compared with mesenchymal stem cells. Level of heparanase was lower in bone marrow (BM) plasma compared with peripheral blood (PB) plasma. BM plasma attenuated heparanase procoagulant activity and level and increased proliferation in osteoblasts and HUVECs compared to PB plasma. In addition, BM plasma increased HUVECs angiogenesis assay (tube-formation) compared with PB.

Journal: Scientific reports

Article Title: Effect of bone marrow blood versus peripheral blood on the hemostatic balance of osteoblasts and endothelial cells.

doi: 10.1038/s41598-025-94942-x

Figure Lengend Snippet: Fig. 8. Graphical summarization. Levels of heparanase, TF, TFPI, and TFPI-2 were reduced in osteoblasts compared with mesenchymal stem cells. Level of heparanase was lower in bone marrow (BM) plasma compared with peripheral blood (PB) plasma. BM plasma attenuated heparanase procoagulant activity and level and increased proliferation in osteoblasts and HUVECs compared to PB plasma. In addition, BM plasma increased HUVECs angiogenesis assay (tube-formation) compared with PB.

Article Snippet: Polyclonal anti-TF, polyclonal anti-TFPI antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Clinical Proteomics, Activity Assay, Angiogenesis Assay